Fig. 4.
a Phenotypes of leaves of Nicotiana benthamiana showing effects of transiently expressed gene constructs: Empty (Empty Vector) and PaO (left) and SGRL and two wild-type alleles of SGR (right) after 144 h; SGR-C, SGR from cv. Cameor; SGR-B, SGR from cv. Birte. b Changes in Fv/Fm over time following infiltration of SGRL, SGR or Empty Vector constructs in comparison to non-infiltrated areas, with images of leaves at each time point shown below. c Chlorophyll (a, b and total) concentration of leaf areas following transient expression for 140 h (see b), with individual Bonferroni statistical analysis for chlorophyll a, b and total as indicated by highlighted letters; within each analysis, different letters (a–d) indicate significant differences; p < 0.05. d Analysis of transiently expressed proteins 144 h after infiltration of PaO, SGR, SGR plus PaO, SGRL or its mutant derivatives, S241stop, M229stop; gels showing total proteins stained (left two panels), blots showing His-tagged proteins (right two panels). Arrows indicate the positions of protein size markers, with their molecular weights indicated in the centre (kDa × 10−3). e Non-denaturing PAGE analysis of proteins from leaf areas infiltrated with PaO, SGR plus PaO, SGRL and Empty (Empty Vector) at 24, 48, 72, 96, 120 and 144 h after infiltration (left to right in every panel); the positions of the major photosystem proteins according to Liu and Last (2015) are indicated on the right hand side