Figure 5.
Effects of TDIF on the morphology of Asplenium × lucrosum. (A–D) Overall morphology of A.× lucrosum plants grown for 3 months in liquid medium containing no additional peptide (A), 100 nM TDIF (B), 1 μM TDIF (C), or 10 μM TDIF (D). (E) comparison of root morphology grown for 5 weeks in liquid culture containing different concentration of TDIF peptides as indicated. (F–I) Cross sections at the middle of the roots grown in control (F,H) or 1 μM TDIF (G,I) medium. Approximate positions for sectioning were illustrated in (E) by arrowheads. The images for (H,I) are magnification of central cylinder in (F,G). Arrows in (H) indicate protoxylem poles. Scale bars: 2 cm in (A–D), 1 cm in (E), 100 μm in (F,G), and 50 μm in (H,I).