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. 2015 Nov 2;6:8776. doi: 10.1038/ncomms9776

Table 2. Media used for growth and motility experiments.

Medium Composition
TB 1% bacto tryptone
  0.5% NaCl
  pH 7.0
   
MBc Difco Marine Broth 2216
  Centrifuged to remove precipitate
   
MotM 10 mM KPO4
  0.1 mM EDTA
  1 μM L-methionine
  10 mM lactic acid
  0.067 mM NaCl
  pH 7.0
   
BsMM 50 mM KPO4
  1.2 mM MgCl2
  0.14 mM CaCl2
  1 mM (NH4)2SO4
  0.01 mM MnCl2
  20 mM sorbitol
  0.02% Bacto tryptone
  50 μg ml−1 L-histidine
  50 μg ml−1 L-methionine
  50 μg ml−1 L-tryptophan
  pH 7.0
   
TMN 50 mM Tris-HCl
  5 mM MgCl2
  5 mM glucose
  300 mM NaCl
  pH 7.5

BsMM, B. subtilis minimal medium; MBc, Marine Broth 2216 (Difco), clarified by centrifugation at 4,500g for 10 min; MotM, E. coli motility medium; TB, tryptone broth; TMN, TMN motility medium.