Table 2. Media used for growth and motility experiments.
Medium | Composition |
---|---|
TB | 1% bacto tryptone |
0.5% NaCl | |
pH 7.0 | |
MBc | Difco Marine Broth 2216 |
Centrifuged to remove precipitate | |
MotM | 10 mM KPO4 |
0.1 mM EDTA | |
1 μM L-methionine | |
10 mM lactic acid | |
0.067 mM NaCl | |
pH 7.0 | |
BsMM | 50 mM KPO4 |
1.2 mM MgCl2 | |
0.14 mM CaCl2 | |
1 mM (NH4)2SO4 | |
0.01 mM MnCl2 | |
20 mM sorbitol | |
0.02% Bacto tryptone | |
50 μg ml−1 L-histidine | |
50 μg ml−1 L-methionine | |
50 μg ml−1 L-tryptophan | |
pH 7.0 | |
TMN | 50 mM Tris-HCl |
5 mM MgCl2 | |
5 mM glucose | |
300 mM NaCl | |
pH 7.5 |
BsMM, B. subtilis minimal medium; MBc, Marine Broth 2216 (Difco), clarified by centrifugation at 4,500g for 10 min; MotM, E. coli motility medium; TB, tryptone broth; TMN, TMN motility medium.