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. 2015 Nov 12;2015:838652. doi: 10.1155/2015/838652

Figure 1.

Figure 1

HER2 overexpression and activation of a lipogenic genetic program. (a-b) Relative expression levels of HER2 and ER mRNAs in the HB4a, HB4aC5.2, and SKBR3 cell lines (a) and levels of FAT/CD36, FABP4, DGAT, and FASN mRNAs in the HB4a and HB4aC5.2 cell lines (b). The experiment was performed in quadruplicate. The PCR reaction was performed in duplicate. ∗ P < 0.001 versus HB4a. (c) Activation of FASN protein in HB4a and HB4aC5.2 cells. Immunoprecipitated proteins were subjected to Western blotting for FASN and β-actin, as controls. CNT: control cells; DHA: cells treated with 100 μM DHA for 72 h; TRAS: cells treated with 15 μg/mL trastuzumab for 72 h.