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Relative expression of mTORC1 pathway markers. (a) Relative expression of SREBP1, LIPIN1, and DEPTOR in HB4a and HB4aC5.2 lines. Data were obtained from an experiment performed in quadruplicate. The real-time PCR reaction was performed in duplicate. ∗ P < 0.001 versus HB4a. (b) Detection of phosphorylated and nonphosphorylated forms of p70S6K (pp70S6K and p70S6K, resp.) by Western blot in HB4a and HB4aC5.2 cells.
