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Replication fork progression is impaired following SETD2 depletion. RCC4 cells were treated with control or SETD2 siRNAs for 48 h, sequentially incubated for 20 min with CldU and IdU, followed by DNA fibre preparation. Representative DNA fibres are shown in: (a) (scale bar: 10 μm). (b) Distribution of replication fork progression rates in control versus SETD2-depleted cells.
