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. 2015 Nov 13;6:8786. doi: 10.1038/ncomms9786

Figure 1. A unique staining signature Ks20.8pos/CD166high/CD117low is eligible for ERpos cell sorting and tracking.

Figure 1

(a) Serial cryostat sections of a normal human breast terminal duct lobular unit (TDLU) stained with immunoperoxidase against Ks20.8 (left) and K18 (right), and counterstained with haematoxylin. Note the characteristic scattered staining pattern with Ks20.8 against the uniform lineage-related staining of luminal cells with K18. Scale bar, 50 μm. (b) Multicolour imaging of normal human breast cryostat sections including acini and ducts from four different biopsies stained for Ks20.8 (red), hormone receptors ER–PR (SP1–SP2, green) and DAPI nuclear stain (blue). The Ks20.8pos compartment encompasses the ER–PR-expressing cells. Scale bar, 50 μm. (c) Multicolour imaging of normal human breast cryostat sections stained for CD166 (3A6, red), CD117 (K44.2, red), hormone receptors ER–PR (green) and DAPI stain (blue). While CD166 is highly expressed in hormone receptor-positive cells, CD117 shows the complementary pattern. Scale bar, 50 μm.