Table 1. List of antibodies used for immunostaining and/or FACS analysis.
| Antibody | Clone | Company/Catalogue No. | Peroxidase | Fluorescence | FACS |
|---|---|---|---|---|---|
| AP2β | — | Santa Cruz, sc-8976 | — | 1:50 | — |
| BCL2 | 124 | Dako, M0887 | — | 1:25 | — |
| CDw75 | LN1 | NeoMarkers, MS-130-P | — | 1:10 | — |
| CD117 | 104D2 | Dako, M7140 | — | 1:50 | — |
| CD117 | K44.2 | Sigma, K0131 | — | 1:50 | — |
| CD117, PE | 104D2 | BD Biosciences, 332785 | — | — | 1:20 |
| CD166 | 3A6 | BioLegend, 343902 | — | 1:50 | — |
| CD166, Alexa Fluor 488 | 3A6 | AbD Serotec, MCA1926A488 | — | — | 1:20 |
| CD271, APC | ME20.4 | Cedarlane, CL10013APC | — | — | 1:50 |
| CD326, PerCP/Cy5.5 | 9C4 | BioLegend, 324214 | — | — | 1:10–1:20 |
| TGFβRII | MM0056–4F14 | Abcam, ab78419 | — | — | 1:20 |
| ERα | SP1 | Labvision, RM-9101-S | 1:25 | 1:10 | — |
| ERα | SP1 | Labvision, RM-9101-R7 | Prediluted, ready to use | — | — |
| ERα | 1D5 | Dako, M7047 | 1:100 | 1:25 | — |
| GATA3 | HG3–31 | Santa Cruz, sc-268 | — | 1:25 | — |
| Keratin 8 | M20 | Abcam, ab9023 | 1:50 | 1:50 | — |
| Keratin 8 | TS1 | Novocastra, NCL-CK8-TS1 | 1:50 | — | — |
| Keratin 20* | Ks20.8 | Dako, M7019 | 1:25 | 1:10 | — |
| Keratin 20* | Ks20.8 | Dako, IR777 | Prediluted, ready to use | — | — |
| Keratin 20* | Ks20.8 | Abcam, ab854 | 1:10 | — | — |
| Keratin 20* | Ks20.8 | Genemed, 61–0018–2 | 1:25 | — | — |
| Keratin 20* | Ks20.8 | Santa Cruz, SC-52320 | 1:25 | — | — |
| Keratin 20 | IT-Ks20.10 | Progen Biotechnik, 61054 | 1:25 | — | — |
| Keratin 20 | CK205 | Novocastra, NCL-CK20–543 | 1:50 | — | — |
| Keratin 8/18 | NCL-5D3 | Abcam, ab90102 | — | 1:50 | — |
| Keratin 14 | LL002 | NeoMarkers, MS-115-P | 1:300 | 1:25 | — |
| Keratin 15 | LHK15 | NeoMarkers, MS-1068-P | — | 1:25 | — |
| Keratin 18 | M9 | Monosan, MON3006 | 1:100 | — | — |
| N-cadherin | 32 | BD Transduction Laboratories, 610920 | — | 1:25 | — |
| PR | SAN27 | Vector Laboratories, VP-P987 | 1:100 | 1:25 | — |
| PR | SP2 | Labvision, RM-9102-S | — | 1:10 | — |
| PR | PgR 636 | Dako, M356901–2 | 1:50 | 1:10 | |
| 67-kDa laminin receptor | MLuC5 | Abcam, ab3099 | — | 1:50 | 1:50 |
*Originally described to recognize cytokeratin 20, but keratin 20 is not expressed in normal human breast14.