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. 2015 Nov 7;5:893–899. doi: 10.1016/j.fob.2015.10.009

Fig. 4.

Fig. 4

MiR-519a regulates the expression of FOXF2 in HCC cells. (A) qRT-PCR and (B) Western blot analysis of FOXF2 expression in Huh7 cells after miR-519a overexpression. Overexpression of miR-519a significantly reduced the levels of FOXF2 mRNA and protein in Huh7 cells. n = three independent experiments; ∗P < 0.05. (C) qRT-PCR and (D) Western blot analysis of FOXF2 expression in SMMC-7721 cells after miR-519a knockdown. Inhibition of miR-519a expression significantly increased the levels of FOXF2 mRNA and protein in SMMC-7721 cells; ∗P < 0.05. (E) Spearman’s correlation analysis demonstrated that miR-519a expression level was inversely correlated with FOXF2 mRNA level in HCC tissues.