Table 1.
Effect of different concentrations of cytokinins namely N6-benzyladenine/Kinetin/2-isoPentenyladenine on shoot regeneration from nodal explants of Morus alba L. variety S-1 in Murashige and Skoog medium after 4 weeks of culture.
| Plant growth regulators (mg/l) | Response of explants (%) | Average no. of shoots per explant⁎ | Average shoot length⁎ (cm) |
|---|---|---|---|
| MS basal medium | 0.00 ± 0.0 | 0.00 ± 0.00l | 0.00 ± 0.00k |
| MS + BA | |||
| 0.3 | 75.4 ± 1.3 | 3.43 ± 0.03c | 3.34 ± 0.03cd |
| 0.5 | 92.1 ± 0.5 | 5.62 ± 0.01a | 4.19 ± 0.01a |
| 1.0 | 83.2 ± 1.1 | 4.16 ± 0.03b | 3.73 ± 0.03b |
| 1.5 | 71.3 ± 1.4 | 3.11 ± 0.03d | 2.31 ± 0.04de |
| 2.0 | 70.5 ± 2.1 | 2.12 ± 0.05f | 2.23 ± 0.01fg |
| MS + Kn | |||
| 0.3 | 56.8 ± 0.5 | 1.21 ± 0.02j | 1.86 ± 0.01i |
| 0.5 | 67.5 ± 1.1 | 2.11 ± 0.03f | 2.12 ± 0.02h |
| 1.0 | 75.2 ± 2.3 | 2.81 ± 0.01e | 2.26 ± 0.01ef |
| 1.5 | 79.2 ± 2.1 | 3.09 ± 0.05d | 3.52 ± 0.01c |
| 2.0 | 72.3 ± 1.3 | 1.90 ± 0.03h | 2.21 ± 0.03fg |
| MS + 2-iP | |||
| 0.3 | 54.3 ± 2.3 | 1.03 ± 0.03k | 1.77 ± 0.01j |
| 0.5 | 61.4 ± 2.1 | 1.19 ± 0.04j | 1.87 ± 0.02i |
| 1.0 | 73.3 ± 1.4 | 2.05 ± 0.02g | 2.24 ± 0.01fg |
| 1.5 | 74.1 ± 3.8 | 1.94 ± 0.11gh | 3.36 ± 0.05cd |
| 2.0 | 71.5 ± 2.4 | 1.42 ± 0.03i | 2.20 ± 0.03g |
N6-benzyladenine (BA), Kinetin (KN), 2-isoPentenyladenine (2-iP); Do, same as above.
⁎
Each value represents the mean ± SE; each experiment consisted of 12–15 replicates and repeated three times. Each mean value followed by the same letter does not differ significantly according to Duncan's Multiple Range Test (p ≤ 0.05).