Table 2.
Effect of different concentrations of auxins namely α-naphthalene acetic acid/Indole-3-butyric acid in combination with optimal concentration of N6-benzyladenine on shoot regeneration form nodal explants of Morus alba L. variety S-1 in Murashige and Skoog medium after 4 weeks of culture.
| Growth regulators (mg/l) | Shoot proliferation (%) | Average no. of shoots per explant⁎a | Average shoot length⁎ (cm) |
|---|---|---|---|
| MS + BA + NAA | |||
| 0.5 + 0.3 0.5 + 0.5 0.5 + 1.0 0.5 + 1.5 0.5 + 2.0 |
84.6 ± 2.91 | 4.86 ± 0.03a | 3.87 ± 0.01a |
| 76.7 ± 3.10 | 4.16 ± 0.02b | 3.55 ± 0.02b | |
| 68.7 ± 1.12 | 3.67 ± 0.04c | 3.35 ± 0.01c | |
| 65.3 ± 2.89 | 2.20 ± 0.04d | 2.95 ± 0.03d | |
| 61.2 ± 2.23 | 1.67 ± 0.01e | 2.97 ± 0.01d | |
| MS + BA + IBA | |||
| 0.5 + 0.3 0.5 + 0.5 0.5 + 1.0 0.5 + 1.5 0.5 + 2.0 |
77.6 ± 3.89 | 4.18 ± 0.01b | 3.36 ± 0.01c |
| 72.6 ± 1.66 | 3.65 ± 0.02c | 3.32 ± 0.01c | |
| 62.2 ± 2.22 | 2.18 ± 0.04d | 2.96 ± 0.02d | |
| 59.4 ± 1.54 | 1.65 ± 0.02e | 2.86 ± 0.02e | |
| 57.1 ± 3.09 | 1.27 ± 0.02f | 1.85 ± 0.03f | |
N6-benzyladenine (BA), α-naphthalene acetic acid (NAA), Indole-3-butyric acid (IBA), Do, same as above.
⁎
Each value represents the mean ± SE; each experiment consisted of 12–15 replicates and repeated three times. Each mean value followed by the same letter does not differ significantly according to Duncan's Multiple Range Test (p ≤ 0.05).