. 2015 Oct 31;7:7–15. doi: 10.1016/j.mgene.2015.10.004

Table 3.

Influence of repeated subculture of nodes in Murashige and Skoog medium supplemented with 0.5 mg/l N⁶-benzyladenine on shoot proliferation of Morus alba L. variety S-1.

Subculture passages	No. of shoots/explant^⁎	Shoot length^⁎ (cm)
First	5.62 ± 0.01^d	4.19 ± 0.01^c
Second	11.39 ± 0.02^c	4.17 ± 0.02^c
Third	17.89 ± 0.02^b	5.16 ± 0.01^b
Fourth	32.27 ± 0.01^a	6.38 ± 0.01^a
Fifth	32.23 ± 0.02^a	6.34 ± 0.01^a

Medium: 0.8% agar-solidified full-strength MS medium + 3% sucrose + 0.5 mg/l BA. Data recorded after 4 weeks of each subculture.

^⁎

Each value represents the mean ± SE of three repeated experiments. Each mean value followed by the same letter does not differ significantly according to Duncan's Multiple Range Test (p ≤ 0.05).