Table 7.
List of primers, their sequences and size of the amplified fragments generated by Inter-simple sequence repeats (ISSR) markers for DNA fingerprinting of micropropagated plants of Morus alba L. variety S-1.
| No. | Primer code | Primer sequence (5′–3′) | Annealing temperature (°C) | No. of scorable loci per primer | Total no. of bands amplified | Size range (bp) |
|---|---|---|---|---|---|---|
| 1. | ISSR-01 | (AG)8T | 50.0 | 4 | 84.00 | 300–1500 |
| 2. | ISSR-02 | (AG)8C | 55.0 | 3 | 63.00 | 600–1500 |
| 3. | ISSR-03 | (AG)8G | 50.0 | 5 | 105.00 | 500–1500 |
| 4. | ISSR-04 | (GA)8T | 45.0 | 3 | 63.00 | 600–2000 |
| 5. | ISSR-05 | (GT)8C | 50.0 | 3 | 63.00 | 600–1300 |
| 6. | ISSR-06 | (TC)8G | 53.0 | 2 | 42.00 | 500–1000 |
| 7. | ISSR-07 | (AC)8T | 50.0 | 2 | 42.00 | 500–700 |
| Total | 22 | 462.00 | ||||