Figure 1. MYC regulates glutamine consumption during adenovirus infection.

(a) NHBE cells were either mock infected, infected with AD WT (wild-type strain of adenovirus 5), or infected with AD ORF1 D68A, and cellular glutamine consumption rates were measured at the indicated time points. (b) (left) Glutamine consumption rates of NHBE cells stably expressing scrambled shRNA (shCTR, blue bars) or MYC shRNA (shMYC, red bars) 24 h post mock infection or infection with AD WT (left). (right) Immunoblotting depicting MYC levels in NHBE cells stably expressing scrambled shRNA (shCTR) or MYC shRNA (shMYC). Tubulin antibody (TUBB) was used to control for loading. (c) Relative miR-23a and mir-23b levels in NHBE cells mock infected, infected with AD WT, or infected with AD ORF1 D68A at the indicated time points post infection. (d) Immunoblotting of lysates from NHBE cells infected with AD WT or AD ORF1 D68A at the indicated times post infection. Whole cell lysates were probed with antibodies towards GLS (glutaminase) and ASCT2 (sodium-dependent neutral amino acid transporter type 2). KGA is the full-length GLS and GAC is the shortened splice variant10. Nuclear lysates were probed with an antibody towards MYC. Tubulin antibody (TUBB) was used to control for loading in the whole cell lysates. For (a–c), error bars denote s.d. (n=3), *P<0.05; **P<0.01. Student's t-test.