Figure 2. Adenovirus infection alters host cell glutamine utilization in a MYC activation-dependent manner.

NHBE cells were labelled with U-¹³C₅-glutamine and mock infected or infected with AD WT or AD ORF1 D68A at an MOI of 1. 24 h post infection, intracellular metabolites were extracted and analysed by LC-MS/MS. (a) Schematic tracing the fate of ¹³C atoms from U-¹³C₅-glutamine in reductive carboxylation. (b) Percentage of ¹³C-labelled isotopomers of intermediates in reductive carboxylation. (c) Percentage of ¹³C-labelled isotopomers of amino acids. (d) Relative levels of non-essential and essential amino acids. (e) Percentage of ¹³C-labelled M2 isotopomers of intermediates in the hexosamine biosynthesis pathway. (f) Relative levels of hexosamine biosynthesis pathway intermediates. For (b–f), error bars denote s.d. (n=3), *P<0.05; **P<0.01. Student's t-test.