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. 2015 Nov 16;6:8883. doi: 10.1038/ncomms9883

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(a) Arrangement of the CO₂-fixation operons encoding form I and form II rubisco in A. ferrooxidans. (b) Native PAGE analysis of purified AfLS and AfM complexes compared to hexadecameric Rhodobacter sphaeroides form I (RsLS) and dimeric Rhodospirillum rubrum form II (RrM) rubisco. (c) Determination of native molecular weight of rubisco enzymes by analytical gel filtration. RpM, Rhodopseudomonas palustris form II rubisco (hexameric)10. Ten microgram of protein was loaded per experiment. (d) Gel-filtration analysis of AfM at varying concentrations in the apo form (E) and in the CABP bound form (ECMC).