Skip to main content
. 2015 Nov 16;6:8883. doi: 10.1038/ncomms9883

Figure 5. The mechanism of CbbQO mediated rubisco activation is conserved.

Figure 5

(a–f) Identification of residues in Q2O2 and both forms of rubisco that affect activase function and the stimulation of ATPase activity. ATPase activity assays of the indicated QO complexes (0.27 μM oligomer) in the presence and absence of active and inactive rubisco complexes (3 μM active sites) (a,c,e) and normalized rubisco activase activity (b,d,f) carried out using mutated Q2O2 (a,b), AfLS (c,d) and AfM (e,f). Protein concentrations used for activase assays: QO complexes, 0.27 μM oligomer; AfLS, 0.3 μM active sites; AfM, 0.1 μM active sites. Activase assays were performed using ECMC complexes. Error bars indicate the mean and s.d. of at least three independent experiments.