Figure 1. Representative structures of stable alternative conformations of the two-domain tandem construct I27–I27 formed on refolding.

(a) Native state structure (based on ref. 52). (b) One of the possible structures of the strand-swapped long-lived misfolded state formed after refolding by dilution from high GdmCl concentration (based on Gō-like simulations)27. Residues 3 of the first domain (blue) and 83 of the second domain (red) are labelled with fluorescent dyes and indicated as orange spheres.