Skip to main content
. Author manuscript; available in PMC: 2016 Nov 2.
Published in final edited form as: Curr Biol. 2015 Oct 22;25(21):2751–2762. doi: 10.1016/j.cub.2015.09.025

Figure 1. Warts localizes to mitotic spindle poles in mitotic S2 cells.

Figure 1

(A) Cells were transfected with full-length Warts tagged with an N-terminal FLAG epitope sequence (FLAG:Wts) and stained with antibodies against FLAG and α-tubulin. To visualize endogenous Mud, untransfected cells were stained with an α-tubulin and Mud antibodies. (B) To depolymerize spindle microtubules, cells were treated with colchicine (12.5 µM) for 2 hours prior to fixation and antibody staining. Yellow arrowheads indicate both γ-tubulin-positive centrosomes, to which neither Wts nor Mud show significant localization. α-tubulin staining indicates successful depolymerization of spindle microtubules; note this channel is not shown in the merge panel.