Table 2. Shell matrix proteins from the shells of P. fucata without blast homology.
| Shell layer | Protein ID | Protein name | Domain | pI | MW kDa |
|---|---|---|---|---|---|
| P, N | pfu_aug1.0_10759.1_31979.t1 | PNU1 | ChtBD2 | 5.69 | 98.2 |
| P, N | pfu_aug1.0_14699.1_32469.t1 | PNU2 | D-rich | 4.85 | 43.6 |
| P, N | pfu_aug1.0_14887.1_32490.t1 | PNU3 | Glyco_hydro_18 | 6.07 | 33.8 |
| P, N | pfu_aug1.0_17316.1_18451.t1 | PNU4 | vWA | 4.84 | 7.12 |
| P, N | pfu_aug1.0_287428.1_50307.t1 | PNU5 | vWA | 8.96 | 17.1 |
| P, N | pfu_aug1.0_4881.1_67066.t1 | PNU6 | D-rich | 3.51 | 21.4 |
| P, N | pfu_aug1.0_75094.1_56521.t1 | PNU7 | – | 5.40 | 13.8 |
| P, N | pfu_aug1.0_7598.1_38581.t1 | PNU8 | – | 9.86 | 11.6 |
| P, N | pfu_aug1.0_86755.1_42180.t1 | PNU9 | – | 8.20 | 9.6 |
| P | pfu_aug1.0_1065.1_36810.t1 | PU1 | Cu2_monooxygen | 9.49 | 53.6 |
| P | pfu_aug1.0_10761.1_31980.t1 | PU2 | Glyco_hydro_18 | 8.85 | 58.5 |
| P | pfu_aug1.0_11437.1_03076.t1 | PU3 | Fibronectin type 3 | 5.82 | 43.2 |
| P | pfu_aug1.0_12760.1_53855.t1 | PU4 | vWA+ CCP | 7.74 | 45.0 |
| P | pfu_aug1.0_13143.1_39453.t1 | PU5 | Fibronectin type 3 | 4.90 | 33.2 |
| P | pfu_aug1.0_155246.1_57288.t1 | PU6 | Fibronectin type 3 | 9.81 | 16.0 |
| P | pfu_aug1.0_164724.1_21334.t1 | PU7 | – | 11.6 | 29.0 |
| P | pfu_aug1.0_1843.1_37145.t1 | PU8 | ChtBD2+CCP+ Laminin_G_3 | 5.13 | 21.8 |
| P | pfu_aug1.0_20998.1_69384.t1 | PU9 | – | 5.14 | 9.3 |
| P | pfu_aug1.0_24840.1_62334.t1 | PU10 | Zn2+ binding + CCP | 4.71 | 75.3 |
| P | pfu_aug1.0_289511.1_28801.t1 | PU11 | – | 9.43 | 21.5 |
| P | pfu_aug1.0_853.1_22356.t1 | PU12 | EGF | 8.30 | 38.4 |
| P | pfu_aug1.0_9037.1_60542.t1 | PU13 | – | 7.89 | 9.1 |
| P | pfu_aug1.0_9053.1_67966.t1 | PU14 | Q-rich | 10.2 | 77.3 |
| P | pfu_aug1.0_9430.1_38910.t1 | PU15 | Fibronectin type 3 | 5.12 | 18.1 |
| P | pfu_aug1.0_154829.1_06667.t1 | PU16 | Fibronectin type 3 | 9.63 | 13.6 |
| N | pfu_aug1.0_12674.1_61081.t1 | NU1 | – | 4.82 | 5.28 |
| N | pfu_aug1.0_18113.1_25698.t1 | NU2 | Zf-Tim10_DDP | 8.25 | 70.1 |
| N | pfu_aug1.0_2111.1_22872.t1 | NU3 | – | 7.98 | 29.2 |
| N | pfu_aug1.0_21286.1_69411.t1 | NU4 | – | 8.15 | 17.1 |
| N | pfu_aug1.0_2323.1_15782.t1 | NU5 | ChtBD2, GS-rich | 4.69 | 184.3 |
| N | pfu_aug1.0_23607.1_62238.t1 | NU6 | – | 8.48 | 7.93 |
| N | pfu_aug1.0_24634.1_54990.t1 | NU7 | -GSM-rich | 11.79 | 35.4 |
| N | pfu_aug1.0_28.1_57930.t1 | NU8 | – | 8.76 | 54.8 |
| N | pfu_aug1.0_4561.1_44973.t1 | NU9 | – | 8.76 | 46.5 |
| N | pfu_aug1.0_954.1_58317.t1 | NU10 | Laminin_G_2 | 9.62 | 17.8 |
Aberration: P=prismatic layer, N=nacreous layer; ‘P, N’ means that the SMPs were found in both the layers; Pfu=Pinctada fucata, Pmar=Pinctada margaritifera, Pmax=Pinctada maxima, Cgi= Crassostrea gigas; CA=Carbonic anhdyrase; EGF=Epidermal growth factor-like; VWA= von Willebrand factor (vWF) type A domain; ChtBD2=Chitin binding domain 2; CCP/SCR/SUSHI =The complement control protein; TIMP=Tissue inhibitor of metalloproteinase; SAM=sterile alpha motif; Zf-Tim10_DDP= Zinc finger (Znf) domain. “-” represents undetected. Notes: The protein ID is the gene ID in the draft genome of Pinctada fucata, which is available at http://marinegenomics.oist.jp/genomes/viewer?project_id=20¤t_assembly_version=ver1.0.