Table 2. SAXS-derived hydrodynamic parameters.
Rg (Å) | Dmax (Å) | Porod's volume (Å3) | Estimated MW (kDa) | Theoretical MW (kDa) | |
---|---|---|---|---|---|
WT-APO | 45.1±0.2 | 158 | 401.504 | 241 | 228 (Tetramer) |
WT-GMP | 44.0±0.3 | 154 | 367.818 | 220 | |
WT-GDP | 49.7±0.2 | 159 | 807.586 | 485 | 456 (Octamer) |
WT-GTP | 49.6±0.3 | 163 | 790.699 | 475 | |
ΔBateman-APO | 38.2±0.2 | 128 | 288.670 | 173 | 176 (Tetramer) |
ΔBateman-GMP | 36.8±0.4 | 121 | 298.568 | 179 |
MW, molecular weight; SAXS, small angle X-ray scattering.
Hydrodynamic parameters estimated from the SAXS data in the presence or absence of 5 mM guanine nucleotides. Rg values are given with s.e.