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. 2015 Nov 26;5:17172. doi: 10.1038/srep17172

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(a) The hatching rate is directly related to the concentration of pESNT-PBase mRNA injected. The hatching rate was significantly reduced by increasing the microinjection concentration. (b) Statistical analysis of the transposition efficiency indicates that 200 ng/μL of pESNT-PBase mRNA is the optimal concentration for obtaining the highest transposition efficiency and a moderate hatching rate (ESNT-PB-200a series transgenic strains). (c,d) The TALE-PBase fusion can significantly enhance the transposition frequency and maintain transposition at a high level (ESNT-PB-200a, ESNT-PB-200b and ESNT-PB-HSA), even using a larger PB transposon plasmid and in a new silkworm strain. *P < 0.05, **P < 0.01, using a significance test for percentage of two samples.