Table 1. TALE-mediated piggyBac transposition efficiency in P50 strain.
| Transgenic strain | Injected mRNA/concentration (ng/μL) | Microinjected embryos | Hatched embryos (%) | G1 generation broods | Examined G1 broods | EGFP-positive G1 broods (From examined broods) | Percentage of transposition efficiency (%) |
|---|---|---|---|---|---|---|---|
| ESNT-PB-75 | pESNT-PBase/75 | 770 | 192 (24.9) | 89 | 47 | 3 | 6.4 |
| ESNT-PB-150 | pESNT-PBase/150 | 1100 | 227 (20.6) | 83 | 56 | 18 | 32.1 |
| ESNT-PB-200a | pESNT-PBase/200 | 850 | 71 (8.4) | 58 | 58 | 37 | 63.8 |
| ESNT-PB-400 | pESNT-PBase/400 | 1450 | 44 (3.0) | 17 | 17 | 5 | 29.4 |
| ESNT-PB-200b | pESNT-PBase/200 | 900 | 75 (8.3) | 36 | 36 | 23 | 63.9 |
| ESN-PB-200 | pESN-PBase/200 | 900 | 203 (22.6) | 110 | 48 | 8 | 16.7 |
| ES-PB-200 | pES-PBase/200 | 800 | 93 (11.6) | 119 | 51 | 7 | 13.7 |
The microinjected PB transposon plasmid was pB3 × P3EGFP and the concentration was 300 ng/μL.