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| Transgenic strain | Injected mRNA or DNA/concentration (ng/μL) | Microinjected embryos | Hatched embryos (%) | G1 generation examined broods | DsRed -positive G1 broods | Percentage of transposition efficiency (%) |
|---|---|---|---|---|---|---|
| ESNT-PB-HSA | pESNT-PBase/200 | 1100 | 234 (21.3) | 103 | 56 | 54.4 |
| PB-HSA | PBase (DNA)/200 | 1100 | 258 (23.4) | 116 | 21 | 18.1 |
The microinjected PB transposon plasmid was pB3 × P3DsRed-FLHSA and the concentration was 200 ng/μL.
