Figure 4.

(A) Hemolytic activity of the peptides. Hemolytic activity was evaluated by incubating individual peptide in serial 2-fold dilutions with freshly isolated human erythrocytes in the absence (dCATH (a)) or presence (dCATH (b)) of 10% FBS at 37 °C for 2 h, followed by measuring the released hemoglobin at 405 nm. No FBS was added in the hemolytic activity of melittin (*P < 0.05; **P < 0.01; by unpaired t test. The blue * indicates the difference between melittin and dCATH (a), the red ones means the difference between dCATH (a) and dCATH (b)). (B) Cytotoxic activity of the peptides. HaCat cells were used to evaluate the toxicity of the peptides to mammalian cells, and measured the released MTT at 492 nm. All the tests were performed three times (*P < 0.05; **P < 0.01; by unpaired t test).