Figure 1.

Cresyl violet (CV) staining (the 1^st and 4^th columns), neuronal nuclei (NeuN) immunohistochemistry (the 2^nd and 5^th columns) and fluoro-Jade B (F-J B) histofluorescence staining (the 3^rd and 6^th columns) in the hippocampus of the ischemia only (left three columns) and ischemic preconditioning + ischemia (right three columns) groups after sham operation (A – F) and at 5 days (d; G – L) after transient cerebral ischemia.

In the ischemia only group, a few CV⁺ neurons (arrows), NeuN⁺ neurons (arrows) and many F-J B⁺ cells (asterisk) were found in the SP at 5 d after transient ischemia. However, the distribution patterns of CV⁺ and NeuN⁺ neurons and F-J B⁺ cells in the SP were similar to those in the sham-operated (sham) group. SO: Stratum oriens; SP: stratum pyramidale; SR: stratum radiatum. Scale bars: 800 μm (A, D, G, J) and 50 μm (B, C, E, F, H, I, K, L).