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. 2015 Nov 25;15:935. doi: 10.1186/s12885-015-1837-1

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© Goswami and Sharma-walia. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cytokine array analysis of the conditioned media from (a) adherent cells and (b) sphere cultures of HMEC, SUM149PT and SUM1315MO2 cells. Red box in the panel indicates the spot on the array corresponding to OPG. c and d Semiquantitiative densitometric analysis of the OPG in the supernatants obtained from adherent cells and sphere cultures of HMEC, SUM149PT and SUM1315MO2. e Conditioned media from adherent HMEC, SUM149PT and SUM1315MO2 cells were collected and analyzed by OPG ELISA. f Lysates from adherent cells of (a) HMEC, SUM149PT and SUM1315MO2 or (b) HMEC and SUM190PT were Western blotted for OPG, stripped and immunoblotted for (a) GAPDH or (b) β-actin used as loading control. A representative blot from three independent experiments is shown