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. 2015 Nov 25;15:935. doi: 10.1186/s12885-015-1837-1

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© Goswami and Sharma-walia. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effect of OPG on HMVEC-d capillary tube formation. a and d Schematic of the angiogenesis experiment. b and e Photomicrographs showing HMVEC-d cell tube formation with various supernatants in a matrigel coated 96-well plate. Panels showing angiogenesis in the presence of conditioned media obtained from HMEC, SUM149PT cells, OPG depleted SUM149PT conditioned media, SUM1315MO2 cells, OPG depleted SUM1315MO2 conditioned media and recombinant human OPG media. After 16 h incubation, plates were examined for capillary tube formation under an inverted microscope and photographed at 10X magnification. Circles represent branch points/field (connections among cells), and the lines represent length of the capillary tubes. Each assay was done in triplicate and each experiment was repeated three times. c and f Quantitative representation of branch points/field in the presence of the different media combinations. Fold increase in the branch points/field was calculated considering branch points/field in presence of control HMEC media as 1 fold