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. 2015 Nov 25;15:935. doi: 10.1186/s12885-015-1837-1

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© Goswami and Sharma-walia. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effect of Osteoprotegerin on the proliferation of HMEC spheres. a Schematic of the experiment. b Confocal images of control HMEC spheres grown in various media combinations. HMEC cells were seeded in various media in 6 well ultra-low attachment plates as indicated, grown for 8 days, and imaged (magnification, 10X). Results shown are tiled shots of two different fields and the assay was performed in triplicate. c and d OPG increases the proliferation of HMEC spheres. MTT assay results shown in the panel represent the absorption at 570 nm. HMEC spheres grown in various supernatants for 8 days as described in methods. The values correspond to the mean +/− S.D. of three independent experiments