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. 2015 Nov 25;15:935. doi: 10.1186/s12885-015-1837-1

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© Goswami and Sharma-walia. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Effect of OPG on survival kinases. a Western blot analysis of total cell lysates from HMEC, SUM149PT and SUM1315MO2 sphere culture grown for 8 days. RIPA lysates were immunoblotted for P-AKT, P-p44/42, P-GSK3β and P-p65, and normalized with respect to the levels of total protein levels. b RIPA lysates from HMEC spheres grown in different media combinations were immunoblotted for P-p65, P-GSK3β, P-AKT and P-p44/42, and normalized with respect to the total protein levels. The phosphorylation fold has been calculated quantitatively by ImageJ. Actin was used as the loading control