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Sand fly midgut lysates separated by SDS PAGE, blotted and incubated with biotinylated lectins. SDS PAGE and blotting of three midgut lysates from Lutzomyia longipalpis (lon), Phlebotomus papatasi (pap) and P. tobbi (tob) with lectins: concanavalin A (Con A) and Helix pomatia agglutinin (HPA). + reaction with lectin; - preincubation of the lectin with specific sugar (GalNAc for HPA and MetMan for Con A); HPA specifically reacted with L. longipalpis and P. tobbi
