Figure 1. Expression profile of OsCCX2 under abiotic stresses and Ca²⁺ deficiency.

(A) Microarray expression of OsCCX1, OsCCX2 and OsCCX3 under drought, salt and cold conditions. (B) The quantitative PCR validation of OsCCX2 expression under drought (C), salt and (D) exogenous ABA treatments. X-axis denotes different time points and Y-axis depicts the relative expression value in terms of fold change. (E) The microarray expression profile of OsCCX1, OsCCX2 and OsCCX3 under Ca²⁺ deficiency. Among OsCCXs, OsCCX2 shows distinct and differential expression after 5 and 14 days of Ca²⁺ deficiency and 6 hrs of Ca²⁺ resupply. X-axis represents different nutritional treatments and Y-axis represents signal intensity value. (F) The microarray expression profile of OsCCX2 was validated under similar conditions using qPCR. Three biological replicates were used for both microarray and qPCR analysis and their mean ± SD values are plotted. X-axis represents different nutritional treatments and Y-axis represents relative expression values obtained after normalizing the data against maximum microarray expression value. Blue bars represent the expression from microarrays, while red bars represent the qPCR values. Differences between mean values of treatments and controls were compared using Student’s t -tests (^*P≤0.05, ^**P≤0.01).