Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 26;5:17117. doi: 10.1038/srep17117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) OsCCX2 fused with N-terminal of GFP appears as circular pre-vesicles inside the lumen of the cell and seems to be localized to vacuolar membrane. (B) The OsCCX2 fused at the C-terminal of GFP also showed tonoplast localization. (C) Cells transformed with CaMV 35S -GFP were used as a control. Fluorescence was detected under a confocal laser-scanning microscope (wavelength: 488 nm). (D) The GFP-OsCCX2 co-localized completely with globular vesicles as shown by tonoplast markers (vac-rk). GFP fusions to OsCCX2 proteins are shown in green, mCherry vacuole markers are shown in red and overlay of two-mentioned proteins in dark field view. Scale bar = 40 μm.