Table 1.
Specific growth rates of P. pastoris CBS7435 and generated das knock-out strains on different carbon sources. Values represent mean values ± standard deviations of the growth rate during the exponential growth phase determined in biological triplicates.
Strain | Growth rate [h−1] | ||
D-Glucose | Glycerol | Methanol | |
P. pastoris CBS7435 | 0.25 ± 0.03 | 0.19 ± 0.05 | 0.15 ± 0.03 |
P. pastoris CBS7435 Δdas1 | 0.23 ± 0.06 | 0.21 ± 0.04 | 0.12 ± 0.03 |
P. pastoris CBS7435 Δdas2 | 0.23 ± 0.03 | 0.20 ± 0.02 | 0.09 ± 0.01 |
P. pastoris CBS7435 Δdas1 Δdas2 | 0.26 ± 0.03 | 0.20 ± 0.04 | 0.01 ± 4 × 10−3 |