Table 1.

Details about the qPCR methods used in the study

Gene	Function	Primer designation	Primers and probe sequences (5′–3′)	Fragment length (bp)	Reference	% covering with the endosymbiont of R. turanicus b	% covering with the endosymbiont of A. americanum c
IS1111	Insertion sequence	Forward primer Reverse primer Probe	Confidentiala Confidentiala Confidentiala	76	(1)	58 0 0	63d 0 63d
icd	Isocitrate dehydrogenase	Forward primer Reverse primer Probe	GACCGACCCATTATTCCCT CGGCGTAGATCTCCATCCA CGCCCGTCATGAAAAACGTGGTC	139	(2)	84 0 0	0 0 0
p1	Porine	Qp1-F Qp1-R Probe	CGGCGATTGGCGTTTC GGTTGCGGTAATGCCGTTAA AACTGTTCAAAATCCGAAACGAGTCGCA	68	(3)	0 12d 50d	0 0 0
scvA	Chromatin condensation	QscvA-F QscvA-R Probe	TGGAAAGACAAAATGTCCAACAA GGTTAGAAGCACCCGGTCGT ACGTGGAAAAGACCAACG	69	(3)	52d 0 67d	0 0 0
GroEL/htpB	Heat shock protein	HtpB-1 HtpB-2 Probe	TGGCTCAAGCGATTTTGGTT TTATCAATACCCCGTTTCAAATCC AAAGCCGTTATTGCTGGAATGAACCCC	82	(4)	65d 92d 70d	0 0 0

^aThe detailed protocol used for the amplification of IS1111 will be soon published by Sidi-Boumedine et al. (in preparation) and remains meanwhile confidential;

^bGenBank accession number: CP011126;

^cGenBank accession number: CP007541;

^dsequence positions are distant from each other on the endosymbiont complete genome.