Table 1.
Serologic methods applied for the detection of antibodies against 13 infectious agents in roe deer sera
| Cut-off | |||||
|---|---|---|---|---|---|
| Test | Conjugate | Units | Neg | Pos | |
| MAP | iELISA (Pourquier) | G-HRP | SP | <60 | >70 |
| iELISA (IDEXX) | G-HRP | SP | <45 | >55 | |
| BRU | iELISA | G-HRP | IU | <2 | >2 |
| LEPT | MAT | – | – | – | – |
| COX | iELISA | G-HRP | SP | <40 | >40 |
| ANA | IFA | Anti-deer IgG FITC | Titre | <1/64 | >1/64 |
| CHL | iELISA | Anti-bov IgG HRP | SP | <50 | >60 |
| BVDV1 | VNT | – | Titre | >1/10 | <1/10 |
| BHV1 | VNT | – | Titre | >1/2 | <1/2 |
| BTV | cELISA | – | PN | >75 | <66 |
| TBEV | VNT:RFFIT | – | DIL50 | <10 | >15 |
| SBV | VNT | – | Titre | >1/8 | <1/8 |
| TOX | iELISA | Anti-deer IgG HRP | OD per plate | ||
| NEO | iELISA | G-HRP | SP | <41 | >50 |
G-HRP: protein G horseradish peroxidase (all species); FITC: fluorescein isothiocyanate; S/P: sample/positive control optical density ratio at 450 nm (OD450), determined as: 100× (sample OD450 – negative control OD450/average 2 positive controls OD450 – negative control OD450); IU: international units, corresponding to dilutions (standard curve based on six dilutions of a reference serum: 1/270 to 1/8340); PN: percent negativity compared to the negative kit control; DIL50: dilution at which 50% of the virus is neutralised; OD per plate: for each plate separately: mean corrected OD450 of three negative reference sera + 3× standard deviation OD450.
MAP: Mycobacterium avium subsp. paratuberculosis; BRU: Brucella sp.; COX: Coxiella burnetii; LEPT: Leptospira; ANA: Anaplasma phagocytophilum; CHL: Chlamydia abortus; BVDV1: bovine viral diarrhoea virus 1; BHV1: bovine herpesvirus 1; BTV: bluetongue virus; TBEV: tick-borne encephalitis virus; SBV: Schmallenberg virus; TOX: Toxoplasma gondii; NEO: Neospora caninum.