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Heme b and its modifications in TrHb1s. (A) Heme b. (B) Native cross-link in wild-type Synechococcus and Synechocystis GlbN (GlbN-A). (C) Cross-link engineered in GlbN by placing a histidine at position 79 (GlbN-B). (D) Double cross-link engineered in GlbN (GlbN-AB). Amino acid labeling corresponds to that of Synechocystis GlbN.
