Figure 2. Ly6G^highLy6C⁺ MDSCs are the main source of IL-10 during S. aureus orthopedic implant biofilm infection.

Implant-associated tissues were collected from IL-10-GFP reporter mice at the indicated intervals following infection and processed for flow cytometry. The CD45⁺GFP⁺ leukocyte population from S. aureus biofilm infected tissues (A) was gated to identify IL-10-GFP expressing Ly6C⁺ and Ly6G⁺ cells (B). The percentage of CD45⁺GFP⁺Ly6G^highLy6C⁺ MDSCs (C) and CD45⁺GFP⁺ Ly6G⁻Ly6C⁺ monocytes (D) is shown. Results are representative of 2 independent experiments (n = 10 mice/time point). Statistical differences are denoted by asterisks (*P < 0.05, **P < 0.01; unpaired 2-tailed Student’s t test).