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. 2015 Aug 23;98(6):963–974. doi: 10.1189/jlb.2VMA0515-229RR

Figure 8. Ablation of Pellino-3 significantly attenuates endotoxin tolerance induction.

Figure 8.

THP-1 cells stably expressing scrambled, Pellino-1 or Pellino-3 shRNA were differentiated for 72 h with PMA, washed, and pretreated for 20 h with medium or 100 ng/ml LPS. After washing, the cells were treated for 3 h with medium or 100 ng/ml LPS, RNA was prepared, reverse-transcribed, and subjected to RT-qPCR analyses to examine the expression of Pellino-3 (A), TNF-α (B), and HPRT mRNA, using the corresponding gene-specific primers. The data of a representative experiment (n = 3) are depicted. *P < 0.05.