The percentage of CD4+ T cells producing cytokines in CB6F1 mice injected with PBS, recPRAME protein alone, AS15 immunostimulant alone, or recPRAME+AS15 at 2 weeks or 2 months after the fourth injection. AS15 indicates mice injected with the AS15 immunostimulant alone; PBS, mice injected with phosphate-buffered saline; recPRAME, mice injected with the recombinant PRAME protein alone; recPRAME+AS15, mice injected with recPRAME formulated with the AS15 immunostimulant. The dots represent individual percentages of T cells producing cytokines. *Comparison with recPRAME+AS15 group.