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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biomaterials. 2015 Oct 9;74:178–187. doi: 10.1016/j.biomaterials.2015.10.003

FIGURE 1. Osteogenic markers rapidly regress upon the withdrawal of the osteogenic cocktail.

FIGURE 1

A) After 2, 4, and 6 weeks, osteogenic supplements were removed from one group of osteogenically differentiated MSCs (OM/GM). Osteogenic markers were assessed at 0d, +1d, and +5d. B) Representative Alizarin Red S staining of mineralized matrix after 2, 4, and 6 week culture at baseline (0d). C) Bone sialoprotein (IBSP) expression after 4 weeks. D) Normalized alkaline phosphatase (ALP) activity and E) normalized calcium deposition for 2, 4, and 6 weeks of osteogenic differentiation. Chart values represent mean ± SD for n = 3–4; $p<0.001 vs. OM/GM and OM, †p<0.001 vs. OM, *p<0.05 vs. OM/GM all within same day. Chart values for 3 donors span data points and the line signifies the mean.