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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biomaterials. 2015 Oct 9;74:178–187. doi: 10.1016/j.biomaterials.2015.10.003

FIGURE 4. Decellularized matrices (DMs) preserve calcium deposition compared to individual matrix constituents and function partially through ROCK II.

FIGURE 4

A) Normalized calcium deposition by MSCs cultured in osteogenic media for 2 weeks and seeded on tissue culture plastic ((−) DM), decellularized matrices ((+) DM), human fibronectin (h FN), human collagen type I (h Col I (1×) and h Col I (2×), denoting two concentrations of the protein), rat tail collagen type I (r Col I), and human laminin-α4 (h Laminin). B) F-actin staining using rhodamine phalloidin (red) and SYTOX® Green nuclear stain (green) of untreated osteogenically differentiated MSCs and osteogenically differentiated MSCs exposed to 10 µM Y-27632 for 24 hours. Scale bars represent 200 µm. C) Bone sialoprotein (IBSP) expression of untreated osteogenically differentiated MSCs and osteogenically differentiated MSCs exposed to Y-27632 for 24h. Chart values represent mean ± SD for n = 3–4; #p<0.05 vs. (+) DM, †p<0.001 vs. (+) DM, *p<0.01 vs. untreated (−) DM, $p<0.01 vs. untreated (+) DM.