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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Neuroendocrinol. 2015 Dec;27(12):861–871. doi: 10.1111/jne.12326

Figure 4. Effects of tamoxifen treatment on ERβ or ERβ mutant-mediated promoter activity at an ERE or AP-1 site.

Figure 4

Hippocampal-derived (HT-22) cell lines were transiently co-transfected with an (A) ERE-tk-Luciferase or (B) AP-1-tk-Luciferase reporter construct and the wild type ERβ or phospho-mutant ERβ expression vector (S87A, S87E, S105A, S105E). 24 hours following transfection, cells were treated with 100 nM 4-OH-tamoxifen (TAM) or vehicle (0.01% ethanol) for 15 hours. Transfection efficiency was normalized using a second renilla luciferase reporter construct (rLUC) in all experiments. Data are expressed as the mean percent change in fLUC/rLUC compared to empty vector control ± SEM taken from 4 independent experiments with 6 replicates/experiment. Different letters denote statistically significant differences as calculated with two-way ANOVA and Tukey post-hoc analysis (p<0.05).