Figure 5. gukh Is Required for the Synaptic Localization of SCRIB.
(A–C) GUKH immunoreactivity at type I boutons of (A) wild-type, (B) gukhJ3, and (C) gukhJ3E1/Df, showing a decrease in immunoreactivity levels in the gukh mutants.
(D–F) Type I synaptic boutons immunostained with anti-SCRIB antibodies, showing its synaptic localization in (D) wild-type, (E) its abnormal clustering at synapses of gukhJ3E1 mutants, and (F) the rescue of this abnormal clustering in gukh mutants by presynaptic expression of GUKH-C.
(G and H) Anti-FasII staining at (G) wild-type and (H) gukhJ3E1/Df mutant boutons. Note that FasII staining is not altered in gukh mutants. Scale bar, 17 µm.
