Figure 6.

The pea brz mutant can accumulate toxic levels of Fe in its leaves. Some mutants that present constitutive activation of Fe physiological responses, like the pea brz mutant (above; Romera et al., 1996) and the pea dgl mutant (Romera et al., 2014), can accumulate high levels of Fe and other metals in their leaves (Romera et al., 1996, 2014), which causes toxicity symptoms (left). Upon treatment with ethylene inhibitors, the Fe physiological responses are blocked and the concentration of Fe accumulated in the leaves diminish (Romera et al., 1996, 2014; see Supplementary Material). Consequently, the toxicity symptoms disappear (right). The brz plants (25-d-old) were transferred to nutrient solution with 20 μM FeEDDHA (left) or to nutrient solution with 20 μM FeEDDHA and the ethylene inhibitor AOA (at 20 μM; right) during 7 days.