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. 2015 Nov 27;9:461. doi: 10.3389/fncel.2015.00461

FIGURE 2.

FIGURE 2

Adhesion molecule expression within the ischemic brain of SHRs and WKYs assessed by quantitative real time PCR and immunofluorescence. mRNA expression of (A) vascular cell adhesion molecule 1 (VCAM-1) and (B) intercellular adhesion molecule 1 (ICAM-1) mRNA expression. (C–E) Co-staining of ICAM-1 with Solanum tuberosum lectin (STL), glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (Iba1). ICAM-1 is co-expressed by STL+ endothelial cells within the ipsi- and contralateral hemisphere (C), within the parenchymal basement membrane of post-capillary venules adjacent to the ischemic lesion (D) and by Iba1+ myeloid leukocytes within the ischemic lesion (E). mRNA expression of (F) P-selectin (CD62P) and-staining of CD62P and Iba1 (G). Nuclei were counterstained with DAPI; n = 3/3/3/3, data are mean values ± standard deviation. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bars: (C) 10 μm; (D) 10 and 1.5 μm; (E) 10 μm; (G) 10 μm.