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. 2015 Nov 23;12(12):995–999. doi: 10.7150/ijms.13193

Figure 1.

Figure 1

Chronic alcohol consumption does not affect the expression of UCP-2 in the lung or the spleen. UCP-2 protein expression was measured by Western blot analysis in lung and spleen mitochondrial extracts of ethanol treated and control rats. UCP-2 gene expression was analysed by quantitative real-time polymerase chain reaction. Representative Western blot of lung (1A) and spleen (1B) tissue of a pair of ethanol treated and control rats. Dose-response curves for the lung (1C) and the spleen (1D). Results are mean ± SEM (n=8-10). Gene expression of UCP-2 in the lung (1E) and the spleen (1F) of a control- and an alcohol-fed group. Results are mean ± SEM (n=8-12). Chronic alcohol consumption resulted in no increased gene and protein expression of UCP-2 in both analysed organs. The experiments were repeated and similar results were obtained. VDAC served as a control for equal protein loading.