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Equilibrium dialysis Ca2+ binding curve. Equilibrium dialysis-inductively coupled plasma optical emission spectrometry Ca2+ binding curve is plotted as fractional occupancy (mol of bound Ca2+/mol of hCasq1) versus free Ca2+ (mm) for wild type hCasq1 (solid line, ●), D244G (dotted line, ♦), and M87T hCasq1 (dashed line, ■). Error bars represent S.D. of three independent measurements.
