TABLE 1.

Distribution of template species formed by SSB/template DNA ratios corresponding to specific phases of the HsPol γ stimulation profile

The percentage distribution of template forms generated by SSB variants was determined by transmission electron microscopy. Samples representative of the various phases of the stimulation profiles were prepared as described under “Experimental Procedures.” The underlining indicates the predominant template DNA species at a given phase of the stimulation profile.

Stimulation phase (SSB tetramers/100 nt)	No difference	Collapsed/beaded	Collapsed	Partially open	Fully open
HsmtSSB
Limiting mtSSB (1)	77	17	3	4	0
Limiting mtSSB (1.6)	68	18	9	5	0
Initial stimulation (3.2)	6	9	60	25	0
Maximal stimulation (3.8)	0	0	38	55	7
Inhibition (6.4)	0	0	5	41	54
DmmtSSB
Limiting mtSSB (1.2)	63	36	1	0	0
Initial stimulation (1.8)	0	0	62	38	0
Maximal stimulation (2.5)	0	0	35	53	12
Inhibition (3.5)	0	0	0	16	84
Inhibition (7)	0	0	0	0	100
HsmtSSB loop 2,3
Limiting mtSSB (1.1)	90	3	5	2	0
Limiting mtSSB (1.7)	73	11	14	2	0
Initial stimulation (3.4)	8	45	39	8	0
Maximal stimulation (4)	0	0	35	53	12
Inhibition (6.8)	0	0	19	70	11
DmmtSSB loop 2,3
Limiting mtSSB (1.2)	76	20	2	2	0
Initial stimulation (1.8)	26	24	28	22	0
Maximal stimulation (2.5)	0	0	16	60	24
Inhibition (3.6)	0	0	2	27	71
Inhibition (7.2)	0	0	0	0	100
EcSSB
Initial stimulation (0.85)	0	62	34	3	1
Initial stimulation (1.3)	0	21	61	18	0
Maximal stimulation (2.6)	0	0	1	33	66
Plateau (5.1)	0	0	0	0	100
Plateau (7.7)	0	0	0	0	100