TABLE 1.
Oligonucleotide primers used to make constructs
The oligonucleotide primers are depicted from left to right as 5′ to 3′. F indicates forward, and R indicates reverse.
| For in vitro transcription | |
| AscI-KpnI linker | F: CGTGAGTGAGG |
| R: CGCGCCTCACTCACGGTAC | |
| Plsp1 | F: CAAAAAAAGCAGGCTCGCCCATGATGGTGATGATATCTC |
| R: GTACAAGAAAGCTGGGTCCTATTGCTTATCCACAGCAC | |
| Plsp12–67 | F: AAAAAGCAGGCTTGATGGATTCAAGTGAGACAACGAAG |
| R: the same as the one for Plsp1 | |
| Citrine | F: AAAAGCAGGCTCGCCCATGGTCTCCAAG |
| R: GAAAGCTGGGTTTACTACTTGTATAGCTCGTC | |
| Plsp11–70-Citrine | F: the same as the one for Plsp1 |
| R: the same as the one for Citrine | |
| For overexpression in E. coli | |
| cpSecA163−1042 | F: GGGCGGCCGCGAGAACCTGTACTTCCAGGGTTGCTCTCGGAAGAGAAGCACG |
| R: CCGCGGCCGCGGCGCTTGCAATTGAGGATGG | |